beta Actin Monoclonal Antibody (15G5A11/E2), Alexa Fluor 647
货号：MA1-140-A647
规格：50 µl
价格：4050
产品类型：内参抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：其它
荧光染料：Alexa Fluor 647
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类型: | 单抗 | 同型对照： | IgG1 |
免疫原性： | Purified full-length recombinant human beta-actin expressed in E. coli | 用法： | 1:500(WB) |
Background/Target InformationBeta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
beta Actin Antibody (MA1-140-A647) in WBWestern blot analysis of beta-Actin was performed by loading various cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and blocked with Sea Block blocking buffer for at least 1 hour. The membrane was probed with a AlexaFluor647-conjugated beta-Actin monoclonal antibody (Product # MA1-140-A647) at a dilution of 1:500 for over night at 4C on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging system. |
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