| beta Tubulin Loading Control Antibody (MA5-16308) in FlowFlow cytometry analysis of Beta Tubulin in CEM cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Tubulin loading control antibody (Product # MA5-16308) at a dilution of 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.beta Tubulin Loading Control Antibody (MA5-16308) in IFImmunofluorescent analysis of Beta-Tubulin (green) showing staining in the in the cytoskeleton of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Beta-Tubulin loading control antibody (Product # MA5-16308) in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. beta Tubulin Loading Control Antibody (MA5-16308) in IHC (P)Immunohistochemistry analysis of Beta-Tubulin showing staining in the cytoskeleton of paraffin-embedded mouse colon tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Beta-Tubulin loading control antibody (Product # MA5-16308) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. |
| 1.AutophagyA novel autophagy modulator 6-Bio ameliorates SNCA/α-synuclein toxicity."MA516308 was used in western blot to describe the effects of 6-Bio using a preclinical model of Parkinson disease"AuthorsSuresh SN,Chavalmane AK,Dj V,Yarreiphang H,Rai S,Paul A,Clement JP,Alladi PA,Manjithaya R2.Cancer researchGenetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth."MA5-16308 was used in western blot to demonstrate the key role of essential amino acid transport in the control of mTORC1 and tumor growth caused by genetic disruption of the multifunctional CD98/LAT1 complex"AuthorsCormerais Y,Giuliano S,LeFloch R,Front B,Durivault J,Tambutté E,Massard PA,de la Ballina LR,Endou H,Wempe MF,Palacin M,Parks SK,Pouyssegur J3.Experimental eye researchEarly adaptive response of the retina to a pro-diabetogenic diet: Impairment of cone response and gene expression changes in high-fructose fed rats."MA5-16308 was used in western blot to study the adaptive response of the retina to short-term high fructose treatment"AuthorsThierry M,Pasquis B,Buteau B,Fourgeux C,Dembele D,Leclere L,Gambert-Nicot S,Acar N,Bron AM,Creuzot-Garcher CP,Bretillon L4.Shock (Augusta, Ga.)Carvedilol treatment after myocardial infarct decreases cardiomyocytic apoptosis in the peri-infarct zone during cardioplegia-induced cardiac arrest."MA5-16308 was used in western blot to study the protective effects of carvedilol against cardiomyocyte apoptosis in a model of hypoxia-reperfusion injury"AuthorsYeh CH,Chen TP,Wang YC,Lin YM,Fang SW5.Molecular microbiologyInositol 1,4,5-trisphosphate receptor regulates replication, differentiation, infectivity and virulence of the parasitic protist Trypanosoma cruzi."MA5-16308 was used in western blot to identify and characterize novel Trypanosoma cruzi IP3 receptors and their role in virulence and infectivity"AuthorsHashimoto M,Enomoto M,Morales J,Kurebayashi N,Sakurai T,Hashimoto T,Nara T,Mikoshiba K |
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