HDAC1 Polyclonal Antibody

货号：PA1-860

规格：100 µg

价格：5468

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1.	用法：	2.0 µg/mL(ICC);10 µg/mL(IF);1 µg/mL(WB)

Product Specific InformationPA1-860 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.PA1-860 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluoresence, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~62 kDa protein representing HDAC1 from HeLa cell lysate. Immunocytochemical staining of HDAC1 in HeLa cells with PA1-860 results in nuclear staining.The PA1-860 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.Target InformationChromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

HDAC1 Antibody (PA1-860) in IFImmunofluorescent analysis of HDAC1 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC1 polyclonal antibody (Product # PA1-860) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. HDAC1 Antibody (PA1-860) in IHCImmunohistochemistry was performed on normal biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing HDAC1 (Product # PA1-860) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. HDAC1 Antibody (PA1-860) in IFImmunofluorescent analysis of HDAC1 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a HDAC1 rabbit polyclonal antibody (Product # PA1-860), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.The Journal of neuroscience : the official journal of the Society for NeuroscienceHDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia."PA1-860 was used in ChIP assay, immunohistochemistry, and western blot to study the roles of Pax3, Sox10 and Fabp7 in the mechanism by which HDAC-1 and -2 control the specification of neural crest cells into peripheral glia"AuthorsJacob C,Lötscher P,Engler S,Baggiolini A,Varum Tavares S,Brügger V,John N,Büchmann-Møller S,Snider PL,Conway SJ,Yamaguchi T,Matthias P,Sommer L,Mantei N,Suter U2.LeukemiaChromatin modifications induced by the AML1-ETO fusion protein reversibly silence its genomic targets through AML1 and Sp1 binding motifs."PA1-860 was used in ChIP assay to investigate the importance of Sp1 in functional chromatin modifications induced by AML1-ETO"AuthorsMaiques-Diaz A,Chou FS,Wunderlich M,Gómez-López G,Jacinto FV,Rodriguez-Perales S,Larrayoz MJ,Calasanz MJ,Mulloy JC,Cigudosa JC,Alvarez S3.Journal of virologyTax relieves transcriptional repression by promoting histone deacetylase 1 release from the human T-cell leukemia virus type 1 long terminal repeat."PA1-860 was used in chromatin immunoprecipitation to study the effect of HTLV-1 viral transcriptional activator Tax on transcriptional regulation."AuthorsLu H,Pise-Masison CA,Linton R,Park HU,Schiltz RL,Sartorelli V,Brady JN4.Molecular cellSpecificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor."PA1-860 was used in western blot to investigate how different NuRD subunits regulate the PER complex."AuthorsKim JY,Kwak PB,Weitz CJ5.Neurobiology of agingEffect of high-intensity exercise on aged mouse brain mitochondria, neurogenesis, and inflammation."PA1-860 was used in western blot to determine how intensive exercise affects brain bioenergetics, inflammation, and neurogenesis-relevant parameters"AuthorsE L,Burns JM,Swerdlow RH

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17751.htm