类型:

单抗

同型对照：

IgG

免疫原性：

Full length human recombinant protein of human POU5F1 produced in E.coli.

用法：

数据

OCT4 Antibody (MA1-104-D488) in IF

Immunofluorescent analysis of Oct4/Pou5F1 (green) shows nuclear staining in human HEL 11.4 iPS cells, indicating pluripotency. Oct4/Pou5F1 staining (left panel), DAPI staining (middle panel) and an overlay image of Oct4/Pou5F1 with DAPI (right panel) are shown. HEL 11.4 iPS cells were grown on matrigel-coated chamber slides and fixed with 4% formaldehyde for 15 minutes prior to staining. Cells were probed with a DyLight 488-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-D488) in 0.1% Tween-PBS at a dilution of 1:20, and incubated overnight at 4C. Images were taken at 20X magnification.

OCT4 Antibody (MA1-104-D488) in IF

Immunofluorescence analysis of OCT-4 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OCT-4 Mouse Monoclonal Antibody (Product # MA1-104-D488) at 5µg/ml in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows OCT-4 negative cell line HeLa with no signal. Panel f represents control cells with Isotype control to assess background. The images were captured at 60X magnification.

OCT4 Antibody (MA1-104-D488)

Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using OCT-4 Monoclonal Antibody (Product # MA1-104-D488) showed expression of OCT-4 in NTERA-2 and not in HeLa which is reported to be negative model for OCT-4 expression.

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参考文献：

PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons.