| 产品介绍 | | |
| Rat anti Human CD45 antibody, clone YTH24.5recognizes the human leukocyte common antigen CD45, expressed on all cells of haematopoietic origin except for erythrocytes.The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United Kingdom. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Immunogen | Human T lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Human Seroblock | BUF070A | F | 50 Tests |
| 文献 |
| 1.Bindon, C.I.et al.(1985) Therapeutic potential of monoclonal antibodies to the leukocyte-common antigen. Synergy and interference in complement-mediated lysis.Transplantation. 40 (5): 538-44.2.Byeon, Y.et al.(2015) Isolation of nucleated red blood cells in maternal blood for Non-invasive prenatal diagnosis.Biomed Microdevices. 17 (6): 118.3.Gossart, A.et al.(2019) Mitigation of monocyte driven thrombosis on cobalt chrome surfaces in contact with whole blood by thin film polar/hydrophobic/ionic polyurethane coatings.Biomaterials. 217: 119306. |
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