SOX2 Polyclonal Antibody SOX2

货号：PA1-094,PA1-094X

规格：100 µg,20 µL

价格：5467,2148

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Purified

类型:	多抗	同型对照：	IgG
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:100-1:500(ICC);1:100-1:500(IF);1:100(WB);1:100(Flow);5 µg, 1:1000(IP)

Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (PA1-094) in IHC (P)Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated mouse esophagus tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SOX2 polyclonal antibody (Product # PA1-094) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. SOX2 Antibody (PA1-094) in WBWestern blot analysis of SOX2 was performed by loading 50 µg of F9 and negative control NIH3T3 whole cell lysates, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a SOX2 polyclonal antibody (Product # PA1-094) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). SOX2 Antibody (PA1-094) in IFImmunofluorescent analysis of Sox-2 using anti-Sox-2 polyclonal antibody (Product # PA1-094) shows nuclear localization in the human iPS cell line HEL 11.4. Sox-2 staining (red) and an overlay image of Sox-2 with DAPI (pink) is shown. HEL 11.4 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Sox-2 (Product # PA1-094) at a dilution of 1:100 overnight at 4 °C, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification. SOX2 Antibody (PA1-094) in IFImmunofluorescent analysis of NESTIN (green) and SOX2 (red) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse NESTIN monoclonal antibody (green; Product # MA1-110) at a dilution of 1:500, and a Rabbit SOX2 polyclonal antibody (red; Product # PA1-094X) at a dilution of 1:500 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114) and Donkey anti-Rabbit Alexa Fluor 568 (Product # A10042) at a dilution of 1:1000 in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm. Data courtesy of Dr. Zhexing Wen at Emory University. SOX2 Antibody (PA1-094) in IFImmunofluorescent analysis of ZO-1 (red) and SOX2 (grey) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse ZO-1 monoclonal antibody (red; Product # 33-9100) at a dilution of 1:500, a Rabbit SOX2 polyclonal antibody (grey; Product # PA1-094X) at a dilution of 1:500, and a Chicken MAP2 polyclonal antibody (green) at a dilution of 1:1000 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 568 (Product # A10037), Donkey anti-Rabbit Alexa Fluor 647 (Product # A-31573), and Donkey Anti-Chicken Alexa Fluor 488 at a dilution of 1:1000 as well as DAPI (blue; 1:25000) in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm. Data courtesy of Dr. Zhexing Wen at Emory University.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Frontiers in medicineEmbryonic Stem Cell-Like Subpopulations in Venous Malformation.2.Frontiers in surgeryCancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.3.Frontiers in surgeryCharacterization of Cancer Stem Cells in Colon Adenocarcinoma Metastasis to the Liver.4.Scientific reportsAldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations.5.Scientific reportsAldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations.

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17994.htm