SOX2 Monoclonal Antibody (Btjce), eBioscience™

货号：14-9811-82,14-9811-80

规格：100 µg,25 µg

价格：4067,1599

产品类型：流式抗体

品牌：eBioscience

抗原：SOX2

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：Btjce

荧光染料：Purified

类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	5 µg/mL(ICC);5 µg/mL(IF);1 µg/mL(WB)

Product Specific InformationDescription: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunocytochemistry, western blot and ELISA (Fluorochrome-conjugated Btjce is recommended for use in intracellular flow cytometry).Applications Tested: This Btjce antibody has been tested by immunohistochemistry on FFPE human testes at less than or equal to 5 µg/mL. The antibody can be used with either high or low pH antigen retrieval. This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized iPS cells at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (14-9811-82) in IHC (P)Immunohistochemistry on formalin-fixed paraffin embedded human testes, using 5 µg/mL Anti-Human/Mouse Sox2 Purified followed by biotinylated anti-rat IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.SOX2 Antibody (14-9811-82)Knockdown of SOX2 was achieved by transfecting NTERA-2 cells with SOX2 specific siRNAs (Silencer® select Product # s13294, s13296). Western blot analysis (Fig. a) was performed using whole cell extracts from the SOX2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and un-transfected cells (lane 1). The blots were probed with SOX2 Monoclonal Antibody (Btjce), eBioscience™ Antibody (Product # 14-9811-82, 1µg/ml) and Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # PA1-29927, 0.25µg/ml, 1:4000 dilution). Densitometry analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SOX2. KnockDown validation info. SOX2 Antibody (14-9811-82) in ICCImmunocytochemistry on fixed and permeabilized induced pluripotent stem cells using 5 µg/mL Anti-Human/Mouse Sox2 Purified followed by anti-rat IgG Alexa® 488 (courtesy of Dr.A.Firth, Salk Institute.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Nature communicationsBCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.2.eLifeMicropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.3.PloS oneDifferential expression of microRNAs among cell populations in the regenerating adult mouse olfactory epithelium.4.Nature methodsOne-step generation of conditional and reversible gene knockouts.AuthorsAndersson-Rolf A,Mustata RC,Merenda A,Kim J,Perera S,Grego T,Andrews K,Tremble K,Silva JC,Fink J,Skarnes WC,Koo BK5.eLifeDynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17995.htm