| Baseline-ZERO™ DNase* is ideal for use when you need to be certain that ZERO DNA remains. Baseline-ZERO DNase hydrolyzes both double-stranded (ds) and single-stranded (ss) DNA to mononucleotides with the highest efficiency. In the presence of Mg2+, cleavage of each strand of a dsDNA substrate proceeds independently. 当您需要确定ZERO DNA仍然存在时，Baseline-ZERO™DNase *是理想的选择。 Baseline-ZERO DNase可将双链（ds）和单链（ss）DNA水解为单核苷酸，效率最高。 在存在Mg2 +的情况下，dsDNA底物的每条链的切割均独立进行。Baseline-ZERO DNase must be inactivated prior to the addition of Baseline-ZERO DNasetreated RNA to reverse transcription reactions.To inactivate the enzyme, incubate the completed reaction at 65°C for 10 minutes in the presence of 1X Stop Solution. 必须先将Baseline-ZERO DNase灭活，然后再添加经Baseline-ZERO DNase处理的RNA才能逆转录反应。要使该酶失活，请在1X Stop Solution的存在下于65°C孵育10分钟。Baseline-ZERO DNase比常用的牛胰腺DNaseⅠ更有效地消化dsDNA和ssDNA至单核苷酸。在用Baseline-ZERO DNase处理后，甚至检测不到用DNaseⅠ处理后剩余的小DNA寡核苷酸。该酶为RNA RT-PCR或基因表达芯片实验提供了真正的零基线。 |
| 优点： |
| ▪ Digest unwanted dsDNA and ssDNA molecules including small ssDNA such as random hexamers ▪ Use in sensitive applications requiring reverse transcription where any contaminating DNA is unwanted ▪ 消化不需要的dsDNA和ssDNA分子，包括小的ssDNA，例如随机六聚体▪ 用于需要反转录的敏感应用中，不需要任何污染的DNA |
| 组成成分： |
| ▪ Baseline-ZERO™ DNase：-20°C▪ 10X Baseline-Zero™ DNase Reaction Buffer: -20°C▪ 10X Baseline-Zero™ DNase Stop Solution:-20°C |
| 数据： |
| Figure 1. Baseline-ZERO™ DNase removes small oligonucleotides during DNase treatment.Only Baseline™-ZERO DNase removes the small residual oligonucleotides visible at the bottom of the gel. 图1. Baseline-ZERO™DNase在DNase处理过程中去除了小的寡核苷酸。只有Baseline™-ZERO DNase才能除去凝胶底部可见的小的残留寡核苷酸。 |
| 参考文献： |
| 1. Sambrook, J. et al., (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. 2. Kienzle, N. et al., (1996) BioTechniques 20, 612. |
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