Mouse anti Human CD90 antibody, clone F15-42-1
货号：MCA90PET,MCA90PE
规格：25 Tests,100 Tests
价格：1386,3986
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD90
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：F15-42-1
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Human CD90 antibody, clone F15-42-1recognizes the human CD90 cell surface antigen, a ~25 kDa glycoprotein homologous to rat Thy1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by prothymocytes within the thymus. CD90 is also expressed extensively within the brain.Mouse anti Human CD90 antibody, clone F15-42-1 is routinely tested in flow cytometry on the MOLT4 cell line. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Pack Size: 100 TestsReconstitute in 1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.Pack Size: 25 TestsReconstitute with 0.25 ml distilled water |
| Preparation | - MCA90PE: Purified IgG prepared by ion exchange chromatography
- MCA90PET: Purified IgG prepared by affinity chromatography on Protein G
|
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line. |
| Immunogen | Purified human brain Thy-1. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Pack Size: 100 TestsPrior to reconstitution store at +4℃. Following reconstitution store at +4℃.This product should be stored undiluted.DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsPrior to reconstitution store at +4°C.After reconstitution store at +4°C.DO NOT FREEZE. This product should be stored undiluted. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Tests |
| 文献 |
| 1.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.2.McKenzie, J.L. & Fabre, J.W. (1981) Human thy-1: unusual localization and possible functional significance in lymphoid tissues.J Immunol. 126 (3): 843-50.3.Daar, A.S. & Fabre, J.W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas.Lancet. 2 (8244): 434-8.4.Paul, G.et al.(2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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