Endoglin (CD105) Human ELISA Kit/人内皮糖蛋白 (CD105) ELISA试剂盒
货号：EHENG,EHENGX5,EHENGX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 10 pg/mL |
| 检测范围 | 12.35-3000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 5 μL 血浆 5 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CD105 (Endoglin) |
| Protein aliases | CD105, CD105 antigen, Endoglin |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | END, ENG, HHT1, ORW1 |
| Gene ID | (Human) 2022 |
| Gene symbol | ENG |
| UniProt ID | (Human) P17813 |
| The Human Endoglin ELISA quantitates Hu Endoglin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu endoglin.人内皮糖蛋白ELISA可以定量测定人血清，血浆，缓冲液或细胞培养基中人的内皮糖蛋白。 该检测试剂盒将只识别天然和重组的人内皮糖蛋白。Principle of the method 方法原理The Human Endoglin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人内皮糖蛋白固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CD105 (Endoglin is a Type I transmembrane protein) is highly expressed on human vascular endothelial cells and exists on an O- and N-glycosylated homodimer. Up regulation of CD105 expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumors and its gene expression is stimulated by hypoxia. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2. Mutations in the CD105 gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms of CD105 have been found. Diseases associated with CD105 dysfunction include hereditary hemorrhagic telangiectasia.CD105（内皮糖蛋白是I型跨膜蛋白）在人血管内皮细胞上高度表达，并存在于O-和N-糖基化的同型二聚体上。 已经在肿瘤脉管系统和增殖细胞中证明了CD105表达的上调，表明它是与增殖相关的内皮标记。 CD105在内皮细胞上高表达，并在伤口愈合，梗塞和各种肿瘤中促进血管生成，其基因表达受到缺氧的刺激。 CD105以高亲和力与TGF beta 1和3结合，但不与TGF beta 2结合。CD105基因的突变会导致遗传性出血性毛细血管扩张，也称为Osler-Rendu-Weber综合征1，一种常染色体显性遗传的多系统血管发育不良。 已经发现编码CD105的不同同工型的选择性剪接的转录变体。 与CD105功能障碍有关的疾病包括遗传性出血性毛细血管扩张。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20448.htm
QQ:1749072012