SiteClick Qdot 525 Antibody Labeling Kit

货号：S10449

规格：1 kit

价格：7297

产品类型：蛋白标记

品牌：Thermo Fisher

Create a perfectly labeled antibody with the SiteClick™ Qdot® 525 Antibody Labeling Kit. This kit replaces the conventional Qdot® 525 Antibody Conjugation Kit (Q22041MP). Unlike the conventional amine-thiol crosslinker method, SiteClick™ labeling specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction steps are required, and the labeling is consistent and reproducible each time it is performed. Depending upon the label, the resulting SiteClick™ labeled antibody can be used in flow cytometry, fluorescence imaging, or western blot detection.Important Features of the SiteClick™ Qdot® 525 Antibody Labeling Kit:• Contains everything required to label 100 µg of IgG antibody• Easy to follow step-by-step protocol• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugate.• Qdot® 525 labels can be used in confocal or traditional fluoresce microscopy.• In flow cytometry, Qdot® 525 can be excited by the 488 nm line of the argon-ion laser, or alternatively via excitation at 405 nm. This is true of all Qdot® fluorophores.

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