Furin Human ELISA Kit/人Furin ELISA试剂盒
货号：EHFURIN,EHFURINX5,EHFURINX10
规格：96 tests,96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15 pg/mL |
| 检测范围 | 13.72-10,000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Furin |
| Protein aliases | dibasic processing enzyme, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE, FES upstream region, Furin, furin (paired basic amino acid cleaving enzyme), furin, membrane associated receptor protein, proprotein convertase subtilisin/kexin type 3 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | FUR, FURIN, PACE, PCSK3, SPC1 |
| Gene ID | (Human) 5045 |
| Gene symbol | FURIN |
| UniProt ID | (Human) P09958 |
| The Human Furin ELISA quantitates Hu Furin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Furin.人弗林蛋白酶ELISA定量人血清，血浆或细胞培养基中的人弗林。 该检测试剂盒将专门识别天然和重组人弗林。Principle of the method 方法原理The Human Furin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人弗林蛋白酶固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.弗林蛋白酶是一种与膜相关的钙依赖性丝氨酸蛋白酶，属于枯草杆菌蛋白酶样激素原转化酶（PC）家族。该细胞酶家族的成员裂解大多数激素和神经肽前体。通过组成型分泌途径转运的许多其他细胞蛋白，一些病毒蛋白和细菌毒素也被PC靶向成熟。弗林蛋白酶和其他PC家族成员具有相似的结构，包括异质的〜10 kDa氨基末端前区，高度保守的〜55 kDa枯草杆菌蛋白酶样催化结构域和长度和序列异质的羧基末端结构域。这些酶在适当的细胞区室中前区切割后变得具有催化活性。弗林蛋白酶是唯一已知的具有跨膜结构域的PC。靶蛋白的切割发生在弗林蛋白酶共有序列RX（K / R）R的羧基末端。已经表明，酸性肽序列C771PSDSEEDEG780将弗林蛋白酶定位于反式高尔基体网络。该区域内丝氨酸残基的磷酸化调节弗林蛋白酶蛋白的细胞内路由。另外的信号传导域包括指导从细胞表面内化的四肽序列Y759KGL762。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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