Glucagon Human ELISA Kit/人胰高血糖素 ELISA试剂盒
货号：EHGCG,EHGCGX5,EHGCGX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2.5 pg/mL |
| 检测范围 | 2.03-130 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Glucagon |
| Protein aliases | Glicentin, Glicentin-related polypeptide, GLP-1, GLP-1(7-36), GLP-1(7-37), GLP-2, Glucagon, Glucagon-like peptide 1, Glucagon-like peptide 1(7-36), Glucagon-like peptide 1(7-37), Glucagon-like peptide 2, GRPP, OXM, OXY, Incretin hormone, Oxyntomodulin, preproglucagon |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | GCG, GLP1, GLP2, GRPP |
| Gene ID | (Human) 2641 |
| Gene symbol | GCG |
| UniProt ID | (Human) P01275 |
| The Human Glucagon (Hu GCG) ELISA quantitates Hu GCG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GCG.人胰高血糖素（人GCG）ELISA可以定量人血清，血浆或细胞培养基中的人GCG。 该检测试剂盒将专门识别天然和重组人GCG。Principle of the method 方法原理The Human GCG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人GCG固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.胰高血糖素是在胰腺中产生的29个残基的多肽激素（分子量3482）。在小肠和大肠的粘膜中产生的相关激素肠胰高血糖素（或胃泌酸调节素）由胰高血糖素的29个氨基酸序列组成，并在C端延伸了8个其他残基。胰高血糖素的生物活性包括糖原分解，脂解，糖异生和生酮作用，它们与胰岛素作用具有拮抗作用，因此导致血糖水平升高。免疫细胞化学研究表明，胰岛胰高血糖素存在于A或α细胞中，占胰岛细胞总数的15-20％。这些细胞优选位于人胰岛的外围。胰高血糖素型肽残基的病理表现几乎全部伴随肿瘤或胰高血糖素瘤的存在，因为尚未鉴定出胰高血糖素细胞缺乏或增生的状态。胰高血糖素特异性抗体将被证明是应用免疫组织化学技术的细胞和肿瘤标志物，并可用作激素鉴定的分析工具。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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