Angiopoietin 4 Human ELISA Kit/人血管生成素4 ELISA试剂盒
货号：EHANGPT4,EHANGPT4X5,EHANGPT4X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.25 ng/mL |
| 检测范围 | 0.274-200 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Angiopoietin 4 |
| Protein aliases | ANG-3, ANG-4, Angiopoietin-3, Angiopoietin-4 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | ANG3, ANG4, ANGPT4 |
| Gene ID | (Human) 51378 |
| Gene symbol | ANGPT4 |
| UniProt ID | (Human) Q9Y264 |
| The Human Angiopoietin 4 (Hu APO4) ELISA quantitates Hu APO4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APO4.人血管生成素4（Hu APO4）ELISA定量了人血清，血浆或细胞培养基中的Hu APO4。 该检测试剂盒将专门识别天然和重组Hu APO4。Principle of the method 方法原理The Human APO4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人APO4固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. Ang-1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), is a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression is restricted to sites of vascular remodeling. Ang-3 and Ang-4 represent the mouse and human counterparts of the same gene locus. The structural divergence of Ang-3 and Ang-4 cause their divergent functions. Ang-3 and Ang-4 have very different distributions in their respective species, and Ang-3 appears to act as an antagonist while Ang-4 appears to function as an agonist. Ang-3 and Ang-4 share all the main structural characteristics of Ang-1 and Ang-2 and are homologous throughout the signal peptide, N-terminal region, coiled-coil segment and Fibrinogen-like domain.血管生成素-1（Ang-1）是Tie-2的一种分泌配体，Tie-2是在内皮细胞和造血细胞中表达的一种细胞表面受体酪氨酸激酶。 Ang-1是介导血管成熟的血管生成因子，可能参与内皮细胞的发育。 相关蛋白血管生成素2（Ang-2）是Ang-1激活Tie-2的天然拮抗剂。 在成人组织中，Ang-2的表达仅限于血管重塑的部位。 Ang-3和Ang-4代表同一基因座的小鼠和人类对应物。 Ang-3和Ang-4的结构差异导致它们的功能分歧。 Ang-3和Ang-4在其各自的物种中具有非常不同的分布，Ang-3似乎起拮抗剂作用，而Ang-4起激动剂作用。 Ang-3和Ang-4具有Ang-1和Ang-2的所有主要结构特征，并且在信号肽，N端区域，卷曲螺旋节段和纤维蛋白原样结构域中都是同源的。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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