IL-1RA Equine ELISA Kit/马IL-1RA ELISA试剂盒
货号：EEIL1RN,EEIL1RNX5,EEIL1RNX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,30186,53584
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 10 pg/mL |
| 检测范围 | 12.29-3000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 33 μL 血浆 33 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL1RA |
| Protein aliases | IL-1RN, IL-1ra, IRAP, IL1 inhibitor, Interleukin-1 receptor antagonist protein, interleukin-1 receptor antagonist secretory form |
物种（Tested） | 马 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-1RA, IL1RA, IL1RN |
| Gene ID | (Horse) 100034236 |
| Gene symbol | IL1RN |
| UniProt ID | (Horse) O18999 |
| The Equine Interleukin-1 Receptor Antagonist (Eq IL-1RA) ELISA quantitates Eq IL-1RA in equine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Eq IL-1RA.马白介素-1受体拮抗剂（马 IL-1RA）ELISA定量马血清，血浆或细胞培养基中的马 IL-1RA。 该检测试剂盒将专门识别天然和重组马IL-1RA。Principle of the method 方法原理The Equine IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.马IL-1RA固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin 1 receptor antagonist (ILRA) is a protein related to the IL-1 family based on its similarities in amino acid sequence to both IL-1 beta and IL-1 alpha, similarities in gene structure, and collective gene localization. The human gene contains four exons and maps to chromosome 2q13-14. IL-1 RA binds to the IL-1 receptor and blocks IL-1 action through competitive inhibition. In contrast to IL-1, the binding of IL-1RA does not initiate signaling events. The secretion of IL-1 RA is mediated by a classical signal sequence while neither IL-1alpha nor IL-1beta possess such a sequence. The lack of homology between IL-1 RA and IL-1 alpha or IL-1 beta near the N terminus may provide an explanation for the biosynthetic and functional differences between IL-1 RA and the two IL-1 proteins. The production of IL-1RA is stimulated by many substances including adherent IgG, cytokines, and bacterial or viral components. IL-1RA is an important natural anti-inflammatory protein that when missing or just available in inadequate amounts plays a key role in several pathological conditions such as rheumatoid arthritis, colitis, osteomyelitis, periostitis and pustulosis.白介素1受体拮抗剂（ILRA）是一种与IL-1家族相关的蛋白质，基于其与IL-1 beta和IL-1 alpha的氨基酸序列相似性，基因结构相似性和集体基因定位。人类基因包含四个外显子，并定位到2q13-14号染色体。 IL-1 RA与IL-1受体结合，并通过竞争性抑制作用阻断IL-1的作用。与IL-1相反，IL-1RA的结合不会引发信号事件。 IL-1 RA的分泌是由经典信号序列介导的，而IL-1alpha和IL-1beta都不具有这种序列。 IL-1 RA与靠近N末端的IL-1 alpha或IL-1 beta之间缺乏同源性，可能为IL-1 RA与两种IL-1蛋白之间的生物合成和功能差异提供了解释。许多物质刺激IL-1RA的产生，包括粘附的IgG，细胞因子以及细菌或病毒成分。 IL-1RA是一种重要的天然抗炎蛋白，当缺失或数量不足时，它在多种病理状况（例如类风湿性关节炎，结肠炎，骨髓炎，骨膜炎和脓疱病）中起关键作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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