XIAP Human ELISA Kit/人XIAP ELISA试剂盒

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XIAP Human ELISA Kit/人XIAP ELISA试剂盒

货号:EHXIAP,EHXIAPX5,EHXIAPX10

规格:96 tests,5X96 tests,10X96 tests

价格:7238,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

250 pg/mL

检测范围2.46-600 ng/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameXIAP
Protein aliasesBaculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, IAP-3, IAP-like protein, ILP, Inhibitor of apoptosis protein 3, RING-type E3 ubiquitin transferase XIAP, X-linked IAP, X-linked inhibitor of apoptosis protein, X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesAPI3, BIRC4, hIAP-3, hIAP3, IAP-3, IAP3, ILP1, MIHA, XIAP, XLP2
Gene ID(Human) 331
Gene symbolXIAP
UniProt ID

(Human) P98170

The Human XIAP ELISA quantitates Hu XIAP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu XIAP.人XIAP ELISA可以定量人血清,血浆或细胞培养基中的人XIAP。 该检测试剂盒将专门识别天然和重组人 XIAP。Principle of the method 方法原理The Human XIAP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人XIAP固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.ATP合酶在进化过程中极为保守,可以在植物,真菌,细菌和动物中发现。 ATP合酶是一种跨膜蛋白,负责驱动ADP +磷酸与ATP的可逆反应。 由于电子转移,质子穿过膜的通量来完成该反应。 ATP合酶蛋白有两个主要部分; F1 ATP酶(可溶性)和F0 ATP酶(膜嵌入)。 F1部分由α,β,γ,δ和ε亚基组成。 而F0由a,b和c子单元组成。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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