| 产品详细信息Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.Applications Reported: The B3B4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood andtonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia. |
| 1.Journal of immunology (Baltimore, Md. : 1950)B cells from knock-in mice expressing broadly neutralizing HIV antibody b12 carry an innocuous B cell receptor responsive to HIV vaccine candidates."12-0232 was used in Flow cytometry/Cell sorting to demonstrate a new mouse model for testing vaccine candidates for the routine elicitation of broadly neutralising antibodies against HIV."AuthorsOta T,Doyle-Cooper C,Cooper AB,Doores KJ,Aoki-Ota M,Le K,Schief WR,Wyatt RT,Burton DR,Nemazee D2.Frontiers in immunologyGestational Hypothyroxinemia Affects Its Offspring With a Reduced Suppressive Capacity Impairing the Outcome of the Experimental Autoimmune Encephalomyelitis."12-0232 was used in Flow cytometry/Cell sorting to analyse the immune response after experimental autoimmune encephalitis induction in the adult offspring gestated in hypothyroxinemia."AuthorsHaensgen H,Albornoz E,Opazo MC,Bugueño K,Jara Fernández EL,Binzberger R,Rivero-Castillo T,Venegas Salas LF,Simon F,Cabello-Verrugio C,Elorza AA,Kalergis AM,Bueno SM,Riedel CA3.Frontiers in immunologyGestational Hypothyroxinemia Affects Its Offspring With a Reduced Suppressive Capacity Impairing the Outcome of the Experimental Autoimmune Encephalomyelitis."12-0232 was used in Flow cytometry/Cell sorting to analyse the immune response after experimental autoimmune encephalitis induction in the adult offspring gestated in hypothyroxinemia."AuthorsHaensgen H,Albornoz E,Opazo MC,Bugueño K,Jara Fernández EL,Binzberger R,Rivero-Castillo T,Venegas Salas LF,Simon F,Cabello-Verrugio C,Elorza AA,Kalergis AM,Bueno SM,Riedel CA4.BMC research notesThe defect of SFRP2 modulates an influx of extracellular calcium in B lymphocytes."12-0232 was used in Flow cytometry/Cell sorting to suggest that SFRP2 modulates the influx from extracellular calcium in the B cell receptor signaling pathway."AuthorsTokuda Y,Tanaka M,Yagi T,Tashiro K5.International journal of molecular sciencesS1P Signalling Differentially Affects Migration of Peritoneal B Cell Populations In Vitro and Influences the Production of Intestinal IgA In Vivo."12-0232 was used in Flow cytometry/Cell sorting to study the function of S1P signalling in the third B cell lineage."AuthorsKleinwort A,Lührs F,Heidecke CD,Lipp M,Schulze T |
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