TWEAK Human Instant ELISA Kit/人TWEAK一步法ELISA试剂盒
货号：BMS2006INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 9.7 pg/mL | 产品规格： | 128 tests |
检测范围： | 15.6-1,000 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | TWEAK |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 9.2% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 7.9% | 检测抗体偶联物： | Biotin |
| 同名基因： | APO3L, DR3LG, TNLG4A, TWEAK | 基因名称： | TNFSF12 |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Tumor necrosis factor-like WEAK inducer of apoptosis (Hu TWEAK) ELISA quantitates Hu TWEAK in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu TWEAK.人肿瘤坏死因子样凋亡的WEAK诱导剂（Hu TWEAK）ELISA定量了人血清，血浆，细胞培养上清液，尿液，羊水，胆汁或其他体液中的Hu TWEAK。 该检测试剂盒将专门识别天然和重组的Hu TWEAK。Principle of the method 方法原理The Human TWEAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TWEAK夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.TNF相关的凋亡弱诱导剂（TWEAK）是结构相关细胞因子的肿瘤坏死因子超家族（TNFSF）的成员。 与该家族的大多数其他成员一样，TWEAK是一种与细胞表面相关的II型跨膜蛋白，尽管较小的生物活性形式也可以通过在细胞膜附近裂解来产生。 TWEAK具有多种生物学活性，除了刺激细胞凋亡外，还包括刺激细胞生长和血管生成，诱导炎性细胞因子。 TWEAK信号转导途径尚未很好地建立，但它似乎通过TweakR / Fn14发出信号，其方式类似于针对结合缺少死亡域的受体的其他TNFSF成员所描述的方式。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
TWEAK Human Instant ELISA™ KitRepresentative standard curve for Human TWEAK Instant ELISA |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20890.htm
QQ:1749072012