IL-1 beta Human Instant ELISA Kit/人IL-1 β一步法ELISA试剂盒
货号：BMS224INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 0.7 pg/mL | 产品规格： | 128 tests |
检测范围： | 7.8-500 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | IL-1 beta |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 10.3% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 7.6% | 检测抗体偶联物： | Biotin |
| 同名基因： | IL-1, IL1-BETA, IL1F2 | 基因名称： | IL1B |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Interleukin 1 beta (Hu IL-1β ELISA quantitates Hu IL-1β in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-1β.人白介素1β（HuIL-1βELISA）定量检测人血清，血浆，细胞培养上清液或其他体液中的HuIL-1β。该检测试剂盒将只识别天然和重组的HuIL-1β。Principle of the method 方法与哪里The Human IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-1β夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.白介素-1β（IL-1 beta）是一种由单核细胞，巨噬细胞和树突状细胞表达的促炎细胞因子。响应炎症刺激，IL-1β合成为31 kDa的无活性前体，并累积在细胞质中。将前IL-1β切割成活性的17 kDa蛋白需要激活炎性小体，炎性小体是对病原体，应激条件和其他危险信号有反应的多蛋白复合物。炎性体的激活触发将caspase-1前体加工成其活性形式，进而切割pro-IL-1 beta。 IL-1β缺乏经典ER /高尔基体途径的信号序列肽，并通过一种另类和不完全了解的机制与caspase-1一起分泌。尽管IL-1β最常以其活性形式分泌，但在某些生物学条件下可检测到未切割蛋白的分泌。 IL-1 beta通过两个受体IL-1RI和IL-1RII发出信号，两者均与IL-1 alpha共享。 IL-1β活性可以由IL-1受体拮抗剂（IL-1RA）调节，IL-1RA是许多细胞类型产生的蛋白质，通过竞争性抑制作用阻断受体结合。 IL-1β通过触发靶细胞中其他促炎细胞因子的产生并启动对感染和损伤的急性期反应，在先天宿主防御中发挥重要作用。 IL-1β水平升高与许多慢性炎症疾病相关，IL-1β中和抗体具有潜在的治疗价值。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
IL-1 beta Human Instant ELISA™ KitRepresentative standard curve for Human IL-1 beta Instant ELISA |
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