DMT-Phosphate Super Column (O-DMT-2,2'-sulfonyldiethanol-Suc-CPG); 1000 Å
货号：SCG1-5000-1,SCG1-5000-2,SCG1-5000-5
规格：1 µmol,200 nmol,50 nmol
价格：84.42,56.28,42.21
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| This super column is packed with DMT-Phosphate (Sulfonyldiethanol) CPG, which allows for the preparation of oligonucleotides containing a 3' Phosphate group using standard synthesis protocols. After removal of the DMT group from the support and coupling of a phosphoramidite, subsequent cleavage from the support yields a 3' phosphate group. The 1000 Å pore size is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性： |
| ▪ Chemical Name:DMT-Phosphate-CPG; 1000Å▪ Appearance:White Powder |
| 产品用途： |
| Cleavage conditions:Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.ABI 3900 users: Columns should be compatible with standard ABI protocols. However, when using 50 nmol Super Columns, the following changes are recommended:
1) Change the head pressure/purge pressure/chamber pressure to 3.5 psi.
2) An additional oxidation step
3) OPTIONAL (but may help): extension of the coupling time to 20 sec Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is：79.98 |
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