MCPT-1 (mMCP-1) Mouse Uncoated ELISA Kit/小鼠MCPT-1 (mMCP-1) 未包被ELISA试剂盒

货号：88-7503-88

规格：10 x 96 tests

价格：6261

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	120 pg/mL
检测范围	120-15,000 pg/mL
样本类型/体积	血清 100 μL 上清液 100 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -ELISA/ELISPOT包被缓冲液：10X PBS ELISA 包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物溶液：Tetramethylbenzidine (TMB) 底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-86）
储存温度	2-8℃
Protein name	MCPT-1
Protein aliases	Mast cell protease 1, MMCP-1
物种（Tested）	小鼠
试剂盒类型	Uncoated ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	AV080368, Mcp-1, Mcpt1
Gene ID	(Mouse) 17224
Gene symbol	Mcpt1
UniProt ID	(Mouse) P11034

The Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCPT-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠MCPT-1（mMCP-1）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于执行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量小鼠MCPT-1的蛋白质水平。 需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA是测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.MCPT-1是由肠粘膜肥大细胞表达的唯一糜酶，在肠上皮细胞中发现。 尽管它是组成型表达的，并且可以在正常小鼠的血清中检测到，但是肠道中的寄生虫会导致系统性水平在两天内急剧增加，并在感染后两周达到峰值。 它在宿主抵抗肠道寄生虫的防御中起着重要作用，因为缺乏MCPT-1的小鼠在感染后表现出延迟的反应。 在肠道过敏性超敏反应中也观察到MCPT-1水平升高。 尽管其作用机理与rMCPT-II类似，但可能通过在细胞之间的紧密连接处切割蛋白质来增加肠道通透性，但尚未完全了解其作用机理。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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