Rat anti-Mouse IgG1 Secondary Antibody, PE, eBioscience™/大鼠抗小鼠IgG1二抗 , PE ,eBioscience™

货号：12-4015-82

规格：100 µg

价格：1577

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：M1-14D12

荧光染料：PE

抗体类型:	荧光二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The monoclonal antibody m1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 Secondary Antibody (12-4015-82) in Flow Staining of normal human peripheral blood cells with Anti-Human CD4 (RPA-T4, a Mouse IgG1 isotype) (Product # 14-0049-82) followed by 0.125 µg of Anti-Mouse IgG1 PE. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Frontiers in molecular neuroscienceIncreased White Matter Inflammation in Aging- and Alzheimer's Disease Brain.2. Journal of the American Society of Nephrology : JASNHuman Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation.3. Nature communicationsNFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.4.PloS oneAntibody-mediated targeting of the Orai1 calcium channel inhibits T cell function.5.Acta pharmacologica SinicaEnhanced efficacy of CTLA-4 fusion anti-caries DNA vaccines in gnotobiotic hamsters.

技术参数

产品应用 Flow

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