Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 550/山羊抗小鼠IgG (H+L)二抗， DyLight 550

货号：84540

规格：1 mL

价格：2311

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Purified Mouse IgG, whole molecule

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：DyLight 550

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	1:25 - 1:100（Flow）；2 µg/mL (ICC)；2 µg/mL (IF); 1:50-1:2,000 (IHC); Assay Dependent (IP) 1:5,000-1:20,000 (WB)

产品详细信息

Product # 84540 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 84540 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 550 Amax= 562 nm; Emax= 576 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

靶标信息

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (84540) in IF Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary antibody DyLight® 550 (Product # 84540) was performed using HeLa cells stained with alpha Tubulin (Product # 236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL of mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Secondary antibody DyLight® 550 (Product # 84540) was used at concentration of 2 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Secondary Antibody (84540) in IF Immunofluorescent analysis of Phalloidin (green) and PDI (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification. Mouse IgG (H+L) Secondary Antibody (84540) in IF Immunofluorescent analysis of eGFP using either natural fluorescence (green) or an anti-eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a GFP Epitope Tag monoclonal antibody (Product # MA1-952) at a dilution of 1:20 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification.

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参考文献：

1. Disease models and mechanismsSpontaneous shaker rat mutant - a new model for X-linked tremor/ataxia.2. Molecular and cellular biologyDifferential Effects of Hepatocyte Nuclear Factor 4α Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells.3. Molecular cancer therapeuticsLY2606368 Causes Replication Catastrophe and Antitumor Effects through CHK1-Dependent Mechanisms.

技术参数

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