Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Biotin/山羊抗小鼠IgG (H+L)，Superclonal™重组二抗，生物素

货号：A28176

规格：1 mg

价格：2220

产品类型：二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent (ELISA);0.05-1 µg/mL (WB)

产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Secondary Antibody (A28176) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.25 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Biotin conjugate (Product # A28176) at dilutions 0.05 µg/mL (Fig. 1), 0.5 µg/mL (Fig. 2) and 1 µg/mL (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Secondary Antibody (A28176) in WB Endogenous alpha-tubulin (A), beta-actin (B) and GAPDH (C) was detected at 52kDA, 46 KDa and 36kDa using Mouse anti-alpha -tubulin (Product # 32-2500), beta-actin (Product # AM4302) and GAPDH (Product # 39-8600) primary antibody and Biotin conjugated Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, (Product # A28176) (0.4 µg/mL, 1:2500). HRP conjugated Streptavidin (Product # SA1007) was used as the tertiary detector (1:3000). No primary control to assess background (D) and loading control (LC) mouse anti-GAPDH (Product # 39-8600) for A and B, alpha-tubulin for C is shown. Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa human cervical carcinoma cells (lane 1-5) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System. Chemiluminescence was detected using the Pierce™ ECL Western Blotting Substrate (Product # 32106). Mouse IgG (H+L) Secondary Antibody (A28176) in Flow alpha-tubulin and beta-actin in HeLa human cervical carcinoma cells was labeled with Mouse anti-alpha -tubulin (Product # 32-2500) and beta-actin (Product # AM4302) primary antibody and detected using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Biotin conjugate (Product # A28176) (2.5 µg/mL). Shift in the cell scattering pattern was acquired using Attune Acoustic Focusing Cytometer (Product # 4468770).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

产品应用 ELISA;WB

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-21797.htm