Goat anti-Mouse IgM Secondary Antibody, HRP/山羊抗小鼠IgM二抗，HRP

货号：31440

规格：2 mL

价格：4078

产品类型：二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	0.8 mg/mL	用法：	1:500-1:5,000 (ICC);Assay Dependent (IP);1:500-1:5,000 (IHC);1:10,000-1:200,000 (WB)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31440 has been successfully used in Western blot, and ICC applications.Antibody Specificity: This antibody reacts with the heavy chain portion of mouse IgM, based on immunoelectrophoresis. No antibody was detected against IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other speciesRestoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as undiluted liquid. After dilution, do not use for more than one day.To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Country of Origin: USA 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgM Secondary Antibody (31440) in WBWestern blot analysis of HSC70 was performed by loading 50µg of the indicated whole cell lysates and 15µl of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSC70 monoclonal antibody (Product # MA3-014) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Mouse IgM Secondary Antibody (31440) in WBWestern blot analysis of Heat Shock Protein 60 (HSP 60) was performed by loading 50 µg of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP60 monoclonal antibody (Product # MA3-013) at a concentration of 1 µg/mL overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Mouse IgM Secondary Antibody (31440) in IPImmunoprecipitation of HSC70 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500 µg whole cell lysate with 2 µL of HSC70 monoclonal antibody (Product # MA3-014) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Plus Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSC70 monoclonal antibody (Product # MA3-014) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with goat anti-mouse IgM-HRP secondary antibody (Product # 31440) for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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参考文献：

1.Methods in molecular biology (Clifton, N.J.)Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy.2.The FEBS journalNewly synthesized cAMP is integrated at a membrane protein complex signalosome to ensure receptor response specificity.3.Brain structure and functionUsing a novel PV-Cre rat model to characterize pallidonigral cells and their terminations.4.BMC neuroscienceBoundary cap neural crest stem cells homotopically implanted to the injured dorsal root transitional zone give rise to different types of neurons and glia in adult rodents.5.Journal of virologyThe herpes simplex virus 1 Us11 protein inhibits autophagy through its interaction with the protein kinase PKR.

技术参数

产品应用 ICC;IHC;IP;WB;ELISA

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