Rabbit anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488/兔抗小鼠IgG (H+L), Superclonal™重组二抗，Alexa Fluor 488

货号：A27023

规格：1 mg

价格：3306

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 488

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	1-3 µg/mL (Flow);1 µg/mL (ICC);1 µg/mL (IF)

产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据：

Mouse IgG (H+L) Secondary Antibody (A27023) in IF Immunofluorescence analysis of Rabbit anti-Mouse IgG (H+L) Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antibody for 3 hours at room temperature. Rabbit anti-Mouse IgG (H+L) Secondary Antibody Alexa Fluor® 488 conjugate (Product # A27023) was used at a concentration of 1 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Secondary Antibody (A27023) in IF alpha-Tubulin (green) in U20S human osteocarcinoma cells was labeled with Mouse anti-alpha-Tubulin primary antibody (Product # 32-2500) (1:3000), and visualized using Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27023) (0.4 µg/mL, 1:2500). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50). Mouse IgG (H+L) Secondary Antibody (A27023) in ICC For immunofluorescence analysis, iPSC differentiated to Neural Rosettes were fixed and permeabilized for detection of endogenous Nestin using Anti-Nestin mouse monoclonal antibody (Product # MA1-110, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27023, 1:2000). Panel b) shows representative cells that were stained for detection and localization of Nestin protein (green) in the cytoplasm of Neural Rosettes in comparison to iPSC (a).

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参考文献：

1.Scientific reportsCytotrophoblast, Not Syncytiotrophoblast, Dominates Glycolysis and Oxidative Phosphorylation in Human Term Placenta.

技术参数

产品应用 Flow;ICC;IHC;IF

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