Rabbit anti-Mouse IgG, IgM (H+L) Secondary Antibody/兔抗小鼠IgG，IgM(H+L)二抗

货号：31198

规格：2 mg

价格：4059

产品类型：二抗

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

荧光染料：其它

抗体类型:	二抗	同型对照：
浓度：	2.4 mg/mL	用法：	Assay Dependent (Flow/ICC/IHC/IP/ChIP/IF/WB)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31198 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31198 reacts with the heavy chains of mouse IgG and IgM and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.Country of Origin: USA 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.Cell reportsLeukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes.

技术参数

产品应用 Flow;ICC;IHC;IP;ChIP;IF;WB

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