IL-2 Monoclonal Antibody (JES6-5H4), APC

货号：17-7021-81,17-7021-82

规格：50μg,100μg

价格：1649,2892

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：JES6-5H4

荧光染料：APC

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), AP
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.Applications Reported: JES6-5H4 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This JES6-5H4 antibody has been tested intracellular staininf of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will staina cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

数据

IL-2 Antibody (17-7021-81) in FlowMouse splenocytes were stimulated with PMA/Ionomycin in the presence of Monensin, then surface stained with Anti-Mouse CD4 PE (Product # 12-0041-82), followed by intracellular staining with with 0.125 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse IL-2 APC (right). Total viable cells were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Frontiers in immunologyA New Adjuvant MTOM MediatesMycobacterium tuberculosisSubunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2+T Cells."Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry"2.Nature communicationsMolecular architecture and regulation of BCL10-MALT1 filaments."17-7021 was used in Flow cytometry/Cell sorting to develop a model for the assembly and architecture of the CARD11-BCL10-MALT1 signalling complex and how it functions as a signalling hub in T-lymphocytes."AuthorsSchlauderer F,Seeholzer T,Desfosses A,Gehring T,Strauss M,Hopfner KP,Gutsche I,Krappmann D,Lammens K3.The Journal of experimental medicineMannose receptor high, M2 dermal macrophages mediate nonhealingLeishmania majorinfection in a Th1 immune environment."17-7021 was used in Flow cytometry/Cell sorting to study the origin and functional specialisation of dermal macrophages in cutaneous infections."AuthorsLee SH,Charmoy M,Romano A,Paun A,Chaves MM,Cope FO,Ralph DA,Sacks DL4.Nature communicationsTristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses."Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry"5.EBioMedicineA Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation."Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry"

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-22229.htm