Mouse IgG2a kappa Isotype Control (eBM2a), FITC

货号：11-4724-41,11-4724-42

规格：25 Tests,100 Tests

价格：851,1387

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：FITC

类型:	同型肚子好	同型对照：
浓度：	5 µL/Test	用法：	Assay-Dependent (Ctrl)；5 µL (1 µg)/test(Flow)；Assay-Dependent(ICC)；Assay-Dependent(IF)；Assay-Dependent(IHC)

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis, immunocytochemistry, and immunohistochemistry.Applications Tested: The FITC conjugated mouse IgG2a isotype control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and should be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL 1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."11-4724 was used in Immunocytochemistry to indicate the enhanced anti-tumor efficacy of PD-1-deficient chimeric antigen receptor T cells against hepatocellular carcinoma."AuthorsGuo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z2.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."11-4724 was used in Immunocytochemistry to indicate the enhanced anti-tumor efficacy of PD-1-deficient chimeric antigen receptor T cells against hepatocellular carcinoma."AuthorsGuo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z3.Experimental and therapeutic medicineProteomic changes of CD4+/CD25+/forkhead box p3+regulatory T cells in a 30-day rat model of sepsis survival."11-4724 was used in Flow cytometry/Cell sorting to explain the mechanism by which regulatory T-cells (Tregs) naturally recover and indicate that Tregs in the blood and spleen vary."AuthorsJiao Y,Tan S,Xiong J4.Journal of veterinary scienceExtensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells."11-4724 was used in Flow cytometry/Cell sorting to demonstrate that fully characterized fAD-MSCs could be beneficial when considering the use of these cells in feline disease research."AuthorsKim HR,Lee J,Byeon JS,Gu NY,Lee J,Cho IS,Cha SH5.Iranian journal of basic medical sciencesSertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells."11-4724 was used in Flow cytometry/Cell sorting to confirm that effect Sertoli cell condition medium, can differentiate germ cells into bone marrow mesenchymal stem cells."AuthorsMonfared MH,Minaee B,Rastegar T,Khrazinejad E,Barbarestani M

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-22248.htm