CD65 Monoclonal Antibody (VIM8), FITC

货号：11-0659-41,11-0659-42

规格：25 Tests,100 Tests

价格：0,3090

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：VIM8

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgM Isotype Control (11E10), FITC
浓度：	5 µL/Test	用法：	5 μL (0.25 μg)/test(Flow)

产品详细信息Description: This VIM8 antibody reacts with the desialylated asialoganglioside of human CD65. This type 2 fucosylated carbohydrate antigen is expressed on the surface of all myeloid cells during development. Moreover, CD65 is expressed highly on granulocytes and weakly on monocytes in peripheral blood. CD65 has been reported to be a ligand for CD62E (E-Selectin). However, the sialylated form of CD65 (designated CD65s) can be used as a marker for acute myeloid leukemia (AML) as its expression increases as CD34 decreases. Finally, CD65 is important for extravascular infiltration of AML cells.Applications Reported: This VIM8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This VIM8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type 2 fucosylated carbohydrate antigen is expressed on the surface of all myeloid cells during development. Moreover, CD65 is expressed highly on granulocytes and weakly on monocytes in peripheral blood. CD65 has been reported to be a ligand for CD62E (E-Selectin). However, the sialylated form of CD65 (designated CD65s) can be used as a marker for acute myeloid leukemia (AML) as its expression increases as CD34 decreases. Finally, CD65 is important for extravascular infiltration of AML cells.

数据

CD65 Antibody (11-0659-41) in FlowStaining of normal human peripheral blood cells with Mouse IgM Isotype Control FITC (Product # 11-4752-80) (blue histogram) or Anti-Human CD65 FITC (purple histogram). Cells in the granulocyte gate were used for analysis.

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