CD275 (B7-H2) Monoclonal Antibody (MIH12), Functional Grade, eBioscience™/CD275 (B7-H2) 单克隆 (MIH12), 功能抗体, eBioscience™

货号：16-5889-82,16-5889-38,16-5889-025,16-5889-050

规格：100 µg,5 mg,25 mg,50 mg

价格：3386,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD275

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MIH12

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. The MIH12 monoclonal antibody has also been demonstrated to block the co-stimulatory function of B7RP-1 including the inhibition of T-cell proliferation and IL-10 production.Applications Tested: The MIH12 antibody has been tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.仅用于科研。不用于诊断过程。

数据

CD275 (B7-H2) Antibody (16-5889-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD275 (B7-H2) PE.Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

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参考文献：

流式1.Stem cell research and therapyComprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 16-5889-82) in Flow Cytometry"2.Clinical and experimental immunologyPulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 16-5889-82) in Flow Cytometry"功能检测3.OncoimmunologyPolyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS."16-5889 was used in Functional assays to investigate polyfunctionality of CD8(+) T cells relating to AKT activation."AuthorsFranzese O,Palermo B,Di Donna C,Sperduti I,Ferraresi V,Stabile H,Gismondi A,Santoni A,Nisticò P4.Scandinavian journal of immunologyExpression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.AuthorsYoungnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M中和5.OncoimmunologyTumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors."16-5889 was used in Neutralization experiments to investigate the role of T regulatory cells in human solid tumours."

技术参数

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