CD48 (eBio156-4H9 (156-4H9)), Functional Grade, eBioscience™/CD48 单克隆 (eBio156-4H9 (156-4H9)), 功能抗体, eBioscience™

货号：16-0489-81,16-0489-85,16-0489-38,16-0489-025,16-0489-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1156,4204,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD48

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：eBio156-4H9 (156-4H9)

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The eBio156-4H9 monoclonal antibody reacts with the human CD48 antigen, also known as B cell activation marker 1, SLAM family member 2, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. CD2 is the low affinity ligand for CD48.Recent publications have reported differential expression in mice of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for prediction of the primitiveness of hematopoietic progenitors. Expression of CD48 in hematopoietic cells is found on many leukocytes with little to none on granulocytes and absent on platelets and erythrocytes. Expression increases on B cells with activation.Applications Reported: This eBio156-4H9 (156-4H9) antibody has been reported for use in flow cytometric analysis and functional assay.Applications Tested: This eBio156-4H9 (156-4H9) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.仅用于科研。不用于诊断过程。

数据

CD48 Antibody (16-0489-81)The Journal of experimental medicine 2014 -Figure 3. WASH deficiency impairs repopulation capacity of HSCs. (A) Flow cytometry of HSCs in Ctrl and WASH KO mice. Sca-1 + c-Kit + cells in the Lin - and IL-7Ralpha - population were gated out (top left) for Flk2 analysis (bottom left). Percentages of the indicated cells in parent populations were calculated and shown on the right. IgG was gated as a negative control. n = 15 for each group. (B) Survival curves of CD45.1 mice transplanted with BM cells from Ctrl ( n = 20) and KO mice ( n = 21). Lethally irradiated CD45.1 mice were transplanted with BM cells from Ctrl and KO mice (left) and survival rates were calculated (right). Lethally irradiated CD45.1 mice without transfer served as a negative control ( n = 13). (C) Equal amounts of CD45.1 (CFSE labeled) and CD45.2 (Violet labeled) lin - BM cells were transplanted into lethally irradiated CD45.1 mice, followed by flow cytometry 18 h after transplantation. Homing efficiency was calculated as: absolute number of Violet + lin - cells 18 h after transplantation/absolute number of Violet + Lin - cells transplanted. n = 10 per group. (D and E) 5 x 10 6 BM cells from MxCre - ;WASH flox/flox and MxCre + ;WASH flox/flox mice were transplanted into lethally irradiated CD45.1 mice. 8 wk later, poly(I:C) was administrated as described in Materials and methods, followed by flow cytometry of the indicated cells 8 wk after poly(I:C) injection (D). Otherwise, 5 x 10 6 BM cells from CD45.1 mice were transplanted into lethally irradiated

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参考文献：

流式1.The Journal of experimental medicineWASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner."Published figure using CD48 monoclonal antibody (Product # 16-0489-81) in Flow Cytometry"2.PLoS pathogensIL-1β production through the NLRP3 inflammasome by hepatic macrophages links hepatitis C virus infection with liver inflammation and disease."16-0489 was used in Flow cytometry/Cell sorting to identify intrahepatic IL-1β production as a central feature of liver inflammation during HCV infection."AuthorsNegash AA,Ramos HJ,Crochet N,Lau DT,Doehle B,Papic N,Delker DA,Jo J,Bertoletti A,Hagedorn CH,Gale M中和3.Journal of immunology (Baltimore, Md. : 1950)Simultaneous TCR and CD244 signals induce dynamic downmodulation of CD244 on human antiviral T cells."16-0489 was used in Blocking experiments to reveal a two-signal mechanism inducing CD244 internalisation, with a role in modulation of antiviral immune response."AuthorsPacheco Y,McLean AP,Rohrbach J,Porichis F,Kaufmann DE,Kavanagh DG

技术参数

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