CD273 (B7-DC) Monoclonal Antibody (TY25), Functional Grade, eBioscience™/CD273 (B7-DC) 单克隆 (TY25), 功能抗体, eBioscience™

货号：16-5986-82,16-5986-38,16-5986-025,16-5986-050

规格：100 µg,5 mg,25 mg,50 mg

价格：2564,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD273

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：TY25

荧光染料：其它

_ueditor_page_break_tag_

类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ~25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse.Applications Reported: The TY25 antibody has been reported for use in flow cytometric analysis. It has also been reported for inhibition of ligand binding.Applications Tested: The TY25 antibody has been tested by flow cytometric analysis of mouse splenocytes and B7-DC transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt]仅用于科研。不用于诊断过程。

数据

CD273 (B7-DC) Antibody (16-5986-82) in FlowStaining of mouse B7-DC-transfected L5178Y cells with 0.25 µg of Rat IgG2a Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD273 (B7-DC) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4312).Total viable cells were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

流式1.Nature immunologyProduction of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells."Published figure using CD273 (B7-DC) monoclonal antibody (Product # 16-5986-82) in Flow Cytometry"2.Frontiers in cellular neuroscienceTargeting immune co-stimulatory effects of PD-L1 and PD-L2 might represent an effective therapeutic strategy in stroke."16-5986 was used in Flow cytometry/Cell sorting to investigate PD-L1 and PD-L2 expressing cell types that affect T-cell activation post stroke, and how these could be targeted therapeutically."AuthorsBodhankar S,Chen Y,Lapato A,Vandenbark AA,Murphy SJ,Offner H3.Journal of leukocyte biologyDynamics of lung macrophage activation in response to helminth infection."Published figure using CD273 (B7-DC) monoclonal antibody (Product # 16-5986-82) in Flow Cytometry"

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-26397.htm