CD7 Monoclonal Antibody (4H9), PE, eBioscience™

货号：12-0078-42

规格：100 Tests

价格：2055

产品类型：流式抗体

品牌：eBioscience

抗原：CD7

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：4H9

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.CD7 has also been detected onnumerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma.Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cellsand on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics.仅用于科研。不用于诊断过程。

数据

CD7 Antibody (12-0078-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 eFluor® 450 (Product # 48-0047-42) and Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or Anti-Human CD7 PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Cancer cellA Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic Leukemia."12-0078 was used in Flow cytometry/Cell sorting to report that the GEF Vav1 unexpectedly possesses tumor-suppressor functions in immature T cells."AuthorsRobles-Valero J,Lorenzo-Martín LF,Menacho-Márquez M,Fernández-Pisonero I,Abad A,Camós M,Toribio ML,Espinosa L,Bigas A,Bustelo XR2.PloS oneBroad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells."12-0078 was used in Flow cytometry/Cell sorting to investigate the production of patient-specific T cells for the treatment of acquired or inherited immune disorders and for cancer immunotherapy."AuthorsChang CW,Lai YS,Lamb LS,Townes TM

技术参数

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