CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Functional Grade, eBioscience™/CD62L (L-Selectin) 单克隆 (MEL-14), 功能抗体, eBioscience™

货号：16-0621-86,16-0621-38,16-0621-025,16-0621-050

规格：1 mg,5 mg,25 mg,50 mg

价格：5725,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD62L

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：MEL-14

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: The MEL-14 antibody has been reported for use in flow cytometric analysis. MEL-14 has also been reported in blocking of adhesion in functional studies. (Fluorchrome-conjguated MEL-14 is recommended for use in flow cytometry).Applications Tested: The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.仅用于科研。不用于诊断过程。

数据

CD62L (L-Selectin) Antibody (16-0621-86) in FlowStaining of mouse bone marrow with Anti-Mouse CD62L (L-Selectin) FITC (left) or PE (right).Autofluorescence is shown via open histogram.Cells in the myeloid population was used for analysis.

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参考文献：

流式1.Cancer cellAntibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0621-86) in Flow Cytometry"2.Immunology lettersModeling Sjögren's syndrome with Id3 conditional knockout mice."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0621-86) in Flow Cytometry"3.Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0621-86) in Flow Cytometry"Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production."16-0168 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of Vδ2 T cells in HCV-infected patients compared to healthy controls."AuthorsYin W,Tong S,Zhang Q,Shao J,Liu Q,Peng H,Hu H,Peng M,Hu P,Ren H,Tian Z,Zhang D4.Infection and immunityModulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0621-86) in Flow Cytometry"功能检测5.BloodInactivation of heparan sulfate 2-O-sulfotransferase accentuates neutrophil infiltration during acute inflammation in mice."16-0621 was used in Functional assays to show that inactivation of uronyl 2-O-sulfotransferase in endothelial cells results in enhanced neutrophil recruitment in several models of acute inflammation."AuthorsAxelsson J,Xu D,Kang BN,Nussbacher JK,Handel TM,Ley K,Sriramarao P,Esko JD

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