alpha GalCer:CD1d Complex Monoclonal Antibody (L363), Functional Grade, eBioscience™/alpha GalCer:CD1d Complex 单克隆 (L363), 功能抗体, eBioscience™

货号：16-2019-82,16-2019-025,16-2019-050

规格：100 µg,25 mg,50 mg

价格：1992,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：alpha GalCer:CD1d

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：L363

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.Applications Reported: This L363 antibody has been reported for use in functional assays.Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylcermide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.仅用于科研。不用于诊断过程。

数据

alpha GalCer:CD1d Complex Antibody (16-2019-82)Nature communications 2018 -Fig. 4 Gene knockdown effects on surface CD1d and CD1d-lipid complex formation. a Effect on CD1d surface expression assessed by flow cytometry. The figure represents percentage change in mean fluorescence intensity (MFI) as compared to a non-targeting control. Average value from four experiments is shown. b Flow cytometry analysis of surface expression of alphaGalCer-CD1d complexes by J774-CD1d cells (live, singlets) after GalGalCer loading. Normalized MFI compared to non-targeting control, the average of four experiments is shown. Boxes represent the interquartile range; maximum and minimum values are displayed with vertical lines. p -Values are indicated (paired t -test). See also Supplementary Fig. 3 , 4 , 5 and 6

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参考文献：

流式1.Nature communicationsMrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae."Published figure using alpha GalCer:CD1d Complex monoclonal antibody (Product # 16-2019-82) in Flow Cytometry"

技术参数

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