IL-17A Monoclonal Antibody (eBio64CAP17), Functional Grade, eBioscience™/IL-17A 单克隆 (eBio64CAP17), 功能抗体, eBioscience™

货号：16-7178-81, 16-7178-85,16-7178-38,16-7178-025,16-7178-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1080,3058,18561,67500,112500

产品类型：细胞分选

品牌：Alfa aesar

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5-2 µg/mL（ELISA）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The eBio64CAP17 antibody reacts with human IL-17A; the antibody has been reported to cross react with Rhesus monkey IL-17A, as verified by intracellular staining experiments. The eBio64CAP17 antibody is a neutralizing antibody. This antibody has been shown to have no reactivity to human IL-17F. Reactivity to other members of the IL-17 family has not been evaluated. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.Additionally, activated human CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). eBio64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot withH17F10A7 anti-IL17F monoclonal antibody.The eBio64CAP17 has been shown to react to rhesus and marmoset primates.Applications Reported: The eBio64CAP17 antibody has been reported for use as the capture antibody in a human IL-17A ELISA and ELISPOT assay, for neutralization of IL-17A bioactivity, and for intracellular staining of IL-17A-producing cells.Applications Tested: The Affinity Purified eBio64CAP17 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of human IL-17A protein levels, in combination with the biotinylated eBio64DEC17 antibody (13-7179) for detection and recombinant human IL-17A (14-8179) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5 - 2.0 µg µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.The Functional Grade Purified eBio64CAP17 antibody has been tested by LAL assay to verify lowest endotoxin levels and has been tested for neutralization of IL-17Abioactivity and for ELISPOT capture. For in vitro neutralization, the eBio64CAP17 antibody at less than or equal to 63 ng/mL has been found to neutralize by 50% the biological effect of 5 ng/mL human IL-17A, based on induction of IL-6 secretion by the NHDF cell line. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IL-17A Antibody (16-7178-81) in ELISAHuman IL-17A ELISPOT Assay. Left: Normal human peripheral blood cells cultured for 24 hrs (no mitogen). Right: Normal human peripheral blood cells activated with PMA/Ionomycin for 24 hrs.

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参考文献：

流式1.The Journal of clinical investigationP2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes."Published figure using IL-17A monoclonal antibody (Product # 16-7178-81) in Flow Cytometry"2.PloS oneApplication of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population."Published figure using IL-17A monoclonal antibody (Product # 16-7178-81) in Flow Cytometry"3.PloS oneCCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues."Published figure using IL-17A monoclonal antibody (Product # 16-7178-81) in Flow Cytometry"功能检测4.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsTh17 Responses to Collagen Type V, kα1-Tubulin, and Vimentin Are Present Early in Human Development and Persist Throughout Life."16-7178 was used in Functional assays to investigate the basis of the T helper 17-dependent autoimmune responses associated with fibro-obliterative forms of chronic rejection following heart or lung transplantation."AuthorsSullivan JA,Jankowska-Gan E,Hegde S,Pestrak MA,Agashe VV,Park AC,Brown ME,Kernien JF,Wilkes DS,Kaufman DB,Greenspan DS,Burlingham WJ5.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsDifferential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses."16-7178 was used in in vivo experiments to identify agents targeting the P2X7R that might treat Th17-related transplant pathologies, while maintaining Th1-immunity to infection."中和6. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsDifferential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses."16-7178 was used in in vivo experiments to identify agents targeting the P2X7R that might treat Th17-related transplant pathologies, while maintaining Th1-immunity to infection."7.The Journal of biological chemistryInterleukin-17 stimulates C-reactive protein expression in hepatocytes and smooth muscle cells via p38 MAPK and ERK1/2-dependent NF-kappaB and C/EBPbeta activation."Published figure using IL-17A monoclonal antibody (Product # 16-7178-81) in ELISA"

技术参数

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