TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Functional Grade, eBioscience™/TLR4/MD-2 Complex 单克隆 (MTS510), 功能抗体, eBioscience™

货号：16-9924-82,16-9924-38,16-9924-025,16-9924-050

规格：100 µg,5 mg,25 mg,50 mg

价格：3496,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：TLR4/MD-2

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：MTS510

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）；Assay-Dependent（Neu）；Assay-Dependent（FN）

产品详细信息Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.Applications Reported: MTS510 has been reported for use in flow cytometric analysis and inhibition of LPS-induced cytokine production.Applications Tested: The MTS510 antibody has been tested by flow cytometric analysis of mouse peritoneal exudates cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response isdetermined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.仅用于科研。不用于诊断过程。

数据

TLR4/MD-2 Complex Antibody (16-9924-82) in FlowStaining of thioglycolate induced PECs with Anti-Mouse TLR4/MD-2 Complex PE Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

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参考文献：

流式1.ImmunityChanzyme TRPM7 Mediates the Ca2+Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation."Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry"2.The Journal of biological chemistryGreen Tea Polyphenol Epigallocatechin-3-gallate Suppresses Toll-like Receptor 4 Expression via Up-regulation of E3 Ubiquitin-protein Ligase RNF216."Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry"3.PloS oneInefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A."Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry"中和4.Molecular medicine reportsHigh mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4."16-9924 was used in Neutralization experiments to investigate the mechanisms underlying chronic inflammation and the calcification of aortic valve interstitial cells in calcific aortic valve disease."IF5.EMBO molecular medicineIdentification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles."Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Immunofluorescence"

技术参数

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