GM-CSF Monoclonal Antibody (MP1-22E9), Functional Grade, eBioscience™/GM-CSF单克隆 (MP1-22E9), 功能抗体, eBioscience™

货号： 16-7331-81,16-7331-85,16-7331-025,16-7331-050

规格：50 µg,500 µg,25 mg,50 mg

价格：1156,3274,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：GM-CSF

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：MP1-22E9

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1.25 µg/mL（ELISA）；Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: The MP1-22E9 antibody has been reported for use in ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis.Applications Tested: The Functional Grade Purified MP1-22E9 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of GM-CSF bioactivity.The MP1-22E9 antibody at 1.25 µg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse GM-CSF in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.The MP1-22E9 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the biotin MP1-31G6 (13-7332) antibody for detection and recombinant mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated withinterstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).仅用于科研。不用于诊断过程。

数据

GM-CSF Antibody (16-7331-81)Clinical cancer research : an official journal of the American Association for Cancer Research 2018 -Published figure using GM-CSF monoclonal antibody (Product # 16-7331-81)

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参考文献：

流式1.Clinical cancer research : an official journal of the American Association for Cancer ResearchCross-talk between T Cells and Hematopoietic Stem Cells during Adoptive Cellular Therapy for Malignant Glioma.WB2.Journal of the American Society of Nephrology : JASNGM-CSF Promotes Macrophage Alternative Activation after Renal Ischemia/Reperfusion Injury."16-7331 was used in Western Blotting to study GM-CSF promotion of macrophage alternative activation after renal ischemia/reperfusion injury."AuthorsHuen SC,Huynh L,Marlier A,Lee Y,Moeckel GW,Cantley LG功能检测3.Clinical cancer research : an official journal of the American Association for Cancer ResearchCross-talk between T Cells and Hematopoietic Stem Cells during Adoptive Cellular Therapy for Malignant Glioma.4.NaturePulmonary macrophage transplantation therapy."16-7331 was used in Functional assays to identify mechanisms regulating alveolar macrophage population size in health and disease, indicate that GM-CSF is required for phenotypic determination of alveolar macrophages, and support translation of PMT as the first specific therapy for children with hPAP."AuthorsSuzuki T,Arumugam P,Sakagami T,Lachmann N,Chalk C,Sallese A,Abe S,Trapnell C,Carey B,Moritz T,Malik P,Lutzko C,Wood RE,Trapnell BC中和5.OncotargetThe bone marrow microenvironment enhances multiple myeloma progression by exosome-mediated activation of myeloid-derived suppressor cells."16-7331 was used in Blocking experiments to investigate the effect of BMSC-derived exosomes on multiple myeloma BM cells, showing that multiple myeloma progression is enhanced through exosome-mediated activation of myeloid-derived suppressor cells."AuthorsWang J,De Veirman K,De Beule N,Maes K,De Bruyne E,Van Valckenborgh E,Vanderkerken K,Menu E

6.Journal of the American Society of Nephrology : JASN GM-CSF Promotes Macrophage Alternative Activation after Renal Ischemia/Reperfusion Injury. "16-7331 was used in Western Blotting to study GM-CSF promotion of macrophage alternative activation after renal ischemia/reperfusion injury." AuthorsHuen SC,Huynh L,Marlier A,Lee Y,Moeckel GW,Cantley LG

技术参数

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