Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor 488
货号：53-6503-80,53-6503-82
规格：25μg,100μg
价格：2052,4487
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：MF20
荧光染料：Alexa Fluor 488
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor 488 |
| 浓度： | 0.5 mg/mL | 用法： | Assay-Dependent (IHC (F))；5 µg/mL(ICC)；5 µg/mL (IF) |
| 产品详细信息Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.The MF20 has been shown to react to myosin froma variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction. |
| 数据 |
| Myosin 4 Antibody (53-6503-82) in ICCImmunocytochemistry of differentiated C2C12 cells using 5 µg/mL of Mouse IgG2b Isotype Control Alexa Fluor® 488 (Product # 53-4732-80) (left) or 5 µg/mL Anti-Myosin Heavy Chain Alexa Fluor® 488 (right). Myosin is positive in a subpopulation of differentiated cells. Nuclei are counterstained with DAPI. |
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| 参考文献： |
| 1.Scientific reportsTAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice."Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunofluorescence"2.Eye (London, England)Ultrastructure of medial rectus muscles in patients with intermittent exotropia."Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot"3.Journal of molecular and cellular cardiologyOptimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success."53-6503 was used in Immunohistochemistry to conclude that HNGMT produces iCMs more efficiently than previously published methods."4.Circulation researchTargeted Ablation of Periostin-Expressing Activated Fibroblasts Prevents Adverse Cardiac Remodeling in Mice."53-6503 was used in Immunohistochemistry to study the effect of activated cardiac fibroblast ablation on cardiac remodeling."AuthorsKaur H,Takefuji M,Ngai CY,Carvalho J,Bayer J,Wietelmann A,Poetsch A,Hoelper S,Conway SJ,Möllmann H,Looso M,Troidl C,Offermanns S,Wettschureck N5.Scientific reportsTAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice."Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunofluorescence" |
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