The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The HIT8a antibody has been reported for use in flow cytometric analysis.Applications Tested: The HIT8a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Staining of normal human peripheral blood cells with Anti-Human CD8a Biotin followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.

"13-0089 was used in Immunofluorescence to explore the mechanisms behind Aire(+) medullary thymic epithelial cell development."

AuthorsWhite AJ,Nakamura K,Jenkinson WE,Saini M,Sinclair C,Seddon B,Narendran P,Pfeffer K,Nitta T,Takahama Y,Caamano JH,Lane PJ,Jenkinson EJ,Anderson G

Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.

"13-0089 was used in Immunofluorescence to explore the mechanisms behind Aire(+) medullary thymic epithelial cell development."

AuthorsWhite AJ,Nakamura K,Jenkinson WE,Saini M,Sinclair C,Seddon B,Narendran P,Pfeffer K,Nitta T,Takahama Y,Caamano JH,Lane PJ,Jenkinson EJ,Anderson G

3.Cancer science

New mouse model of acute adult T-cell leukemia generated by transplantation of AKT, BCLxL, and HBZ-transduced T cells.

"13-0089 was used in Flow cytometry/Cell sorting to develop methods to explore the role of candidate genes with the HBZ gene in adult T-cell leukemia/lymphoma development, producing a new mouse model."

AuthorsKasugai Y,Yoshida N,Ohshima K,Matsuo K,Seto M,Tsuzuki S