CD8a Monoclonal Antibody (HIT8a), eBioscience™
货号：14-0089-82
规格：100 µg
价格：1136
产品类型：细胞分选
品牌：Thermo Fisher
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 µg/test |
| Description:The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The HIT8a antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: The HIT8a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据 |
CD8a Antibody (14-0089-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a FITC (left) and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis. |
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| 参考文献： |
| 1.The Journal of clinical investigationTransplantation of mouse HSCs genetically modified to express a CD4-restricted TCR results in long-term immunity that destroys tumors and initiates spontaneous autoimmunity."14-0089 was used in Flow cytometry/Cell sorting to study how a translational model of immunotherapy, utilising unique properties of HSC transplantation, has potential for the treatment of metastatic melanoma."AuthorsHa SP,Klemen ND,Kinnebrew GH,Brandmaier AG,Marsh J,Hangoc G,Palmer DC,Restifo NP,Cornetta K,Broxmeyer HE,Touloukian CE2.Clinical immunology (Orlando, Fla.)Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a."14008982 was used in flow cytometry to determine the S. Typhi, S. Paratyphi A, and S. Paratyphi B cross-reactive CD4 positive T cell responses elicited by immunization with Ty21a"AuthorsWahid R,Fresnay S,Levine MM,Sztein MB |
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