Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine5, eBioscience™

货号：15-4301-82

规格：100μg

价格：2271

产品类型：大鼠同型对照

品牌：Thermo Fisher

物种：其它

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBRG1

荧光染料：PE-Cy5

类型:	大鼠同型对照	同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: PE-Cy5 Rat IgG1 Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: This PE-Cy5 Rat IgG1 isotype control antibody should be used at the same concentration as the experimental antibody.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection."15-4301 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection."

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