CD5 Monoclonal Antibody (53-7.3), APC-eFluor 780, eBioscience™

货号：47-0051-80,47-0051-82

规格：25μg,100μg

价格：1132,2892

产品类型：流式抗体

品牌：eBioscience

抗原：CD5

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：53-7.3

荧光染料：APC-eFluor 780

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780, eBioscience
浓度：	0.2mg/mL	用法：	0.125 µg/test (Flow)

Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: This 53-7.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD5 Antibody (47-0051-80) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 PerCP-Cyanine5-5 (Product # 45-0193-82) and 0.06 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.06 µg of Anti-Mouse CD5 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.The Journal of biological chemistryCD36 receptor regulates malaria-induced immune responses primarily at early blood stage infection contributing to parasitemia control and resistance to mortality."47-0051 was used in Flow cytometry/Cell sorting to study the function of CD36 in malaria immunity."2.Journal of virologyHost Tumor Suppressor p18INK4cFunctions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis."47-0051 was used in Flow cytometry/Cell sorting to characterise the role of host p18INK4c in inhibiting gammaherpesvirus reactivation and pathogenesis."3.Nature communicationsNFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells."Published figure using CD5 monoclonal antibody (Product # 47-0051-82) in Flow Cytometry"

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