CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), FITC,

货号：11-0903-82,11-0903-85

规格：100 ug ,500 ug

价格：1029,2677

产品类型：流式抗体

品牌：eBioscience

抗原：CD90.2 (Thy-1.2)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：30-H12

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC
浓度：	0.5 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

数据

CD90.2 (Thy-1.2) Antibody (11-0903-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (left) or 0.06 µg of Anti-Mouse CD90.2 (Thy-1.2) FITC (right). Cells in the lymphocyte gate were used for analysis. CD90.2 (Thy-1.2) Antibody (11-0903-82)Nature communications 2018 -Fig. 6 Quiescent cells act as a reservoir and mobilize to form stem cells. a , b PH3 immuno-staining of sections of CD90/Thy1 cre; mTmG mice shows proliferating cells become detectable in the mesenchyme between the two aspects of the cervical loop after incisor clipping. c , d Detection of EdU incorporating cells that had been labeled during embryonic development from E2.5 to E17.5 and chased for over 6 months, showing a small population of EdU+ cells distributed in a line at then most proximal end of incisor mesenchyme. e Co-staining with CD90/Thy1 and EdU labeled quiescent cells shows CD90/Thy1 cells are located in the mesenchyme adjacent to the EdU labeled cells, red arrows are EdU + and green arrows are CD90/Thy1 + cells. f Co-staining with CD90/Thy1 and Celsr1 shows CD90/Thy1 cells occupy the same location adjacent to the Celsr1 expressing cells. g Triple staining with CD90/Thy1, Celsr1 and EdU shows CD90/Thy1 expressing cells are located adjacent to the EdU and Celsr1 + cells, yellow arrows and h enlarged region showing the co-localization of EdU+ ; Celsr1 + cells. i Immuno-staining of FACS sorted GFP + cells from clipped CD90/Thy1-cre;mTmG mouse incisors with Celsr1 and PH3 on cytospin slides, identifies GFP+ ;Celsr1 + ;PH3 + triple positive (white arrow,) and GFP + ;Celsr1 - ;PH3 - cells (green arrow). Bar 250 um in ( a ), ( e ), ( f ), and ( g ). 50 um in b, 100 um in d and 10 um in ( h ) and ( i )

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiation and induces autoimmunity.2. Nature communicationsA quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.3. Nature communicationsA quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.4. Experimental and therapeutic medicineComparison between curcumin and all-trans retinoic acid in the osteogenic differentiation of mouse bone marrow mesenchymal stem cells.5. ImmunityDifferentiation and Protective Capacity of Virus-Specific CD8+T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

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