CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), Super Bright 436,
货号：62-0900-82
规格：100 µg
价格：3144
产品类型：流式抗体
品牌：eBioscience
抗原：CD90.1 (Thy-1.1)
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：HIS51
荧光染料：Alexa Fluor 488
| 类型: | 单抗 | 同型对照： | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright 436 |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 µg/test(Flow) |
产品详细信息Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser.Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
| 数据 |
CD90.1 (Thy-1.1) Antibody (62-0900-82) in FlowWistar rat splenocytes were stained with CD3 Monoclonal Antibody, APC (Product # 17-0030-82) and 0.125 µg of Mouse IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4724-82) (left) or 0.125 µg of CD90.1 Monoclonal Antibody, Super Bright 436 (right). Total viable cells were used for analysis, as determined by 7-AAD |
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