Recombinant Human Visfatin
货号：PHP201
规格：25 µg
价格：3162
产品类型：细胞因子添加物
品牌：Bio-Rad
| 产品介绍 | | |
| Visfatin is a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide.Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant visfatin expressed inE.coli |
| Reconstitution | Reconstitute with 0.25 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.N.B. Due to solubility reasons, it is recommended that the solution be kept at low pH. |
| Buffer Solution | 10mM HCl |
| Activity | The ED50was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED50for this effect is 15.0-20.0 ng/ml. |
| Preservative Stabilisers | None present |
| Approx. Protein Concentrations | Total protein concentration 0.1 mg/ml after reconstitution |
| Protein Molecular Weight | 52.5 kDa (465 amino acid residues) |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
技术参数 产品应用 FN
产品类型 重组蛋白
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