| 产品介绍 | | |
| Rat anti Mouse CD51 antibody, clone RMV-7recognizes murine CD51, a ~140 kDa alpha subunit of the vitronectin receptor, which is otherwise known as the integrin alpha v chain. CD51 can form heterodimers at the cell surface with a variety of beta integrins including CD29 and CD61.Heterodimers of CD51/CD61 function as a receptor for vitronectin, and a wide array of RGD-containing proteins including fibronectin, fibrinogen, von Willebrand factor, laminin, thrombospondin and osteopontin. CD51/CD61 is primarily expressed on myeloid cells and activated T-cells. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing.Rat anti Mouse CD51 antibody, clone RMV-7 has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity (Takahashiet al.1990). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. |
| Fusion Partners | Spleen cells from immunised SD rats were fused with the cells of the P3X.63Ag8.653 myeloma cell line. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Cultured LAK cells from Balb/c mice. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/m |
| Preservative Stabilisers | None present |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃ only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/10Pack Size: 0.1 mg1/25Pack Size: 0.25 mg | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Takahashi, K.et al.(1990) A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction.J Immunol. 145 (12): 4371-9.2.Piali, L.et al.(1995) CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium.J Cell Biol. 130 (2): 451-60.3.Cui Ret al.(2007) Abrogation of the interaction between osteopontin and alphavbeta3 integrin reduces tumor growth of human lung cancer cells in mice.Lung Cancer. 57 (3): 302-10.3.Takahashi Fet al.(2001) Role of osteopontin in the pathogenesis of bleomycin-induced pulmonary fibrosis.Am J Respir Cell Mol Biol. 24 (3): 264-71. |
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