mouse anti human FC EPSILON R1 alpha:PE
货号：MCA2368PE
规格：100 Tests
价格：3893
产品类型：流式抗体
品牌：Bio-Rad
抗原：Fc Epsilon R1 Alpha
物种：人
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：9E1
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Human Fc epsilon R1 alpha antibody, clone 9E1recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | F | 0.5mg |
| 文献 |
| 1.Vangelista, L.et al.(2002) A minimal receptor-Ig chimera of human FcepsilonRI alpha-chain efficiently binds secretory and membrane IgE.Protein Eng. 15 (1): 51-7.2.Vangelista, L.et al.(2002) Efficient folding of the FcepsilonRI alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1.J Mol Biol. 322 (4): 815-25.3.Vangelista, L.et al.(2005) Membrane IgE binds and activates Fc epsilon RI in an antigen-independent manner.J Immunol. 174 (9): 5602-11. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
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