| 产品介绍 | | |
| Mouse anti Human CD49c antibody, clone 17C6recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
Immunogen | CD49c transfected cell line. |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Fusion Partners | Cells from immunised Balb/c mice were fused with cells of the NS2 mouse myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 5ul of the suggested working dilution to label 106cells in 100ul. |
| 数据 |
| Figure A. Alexa Fluor 647 conjugated Mouse anti Human EGFR (MCA1784A647) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B . Alexa Fluor 647 conjugated Mouse anti Human EGFR (MCA1784A647) and PE conjugated Mouse anti Human CD49c (MCA1948PE). All experiments performed on human peripheral blood gated on live single cells, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:PE | MCA928PE | Flow | 100 Test |
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| 文献 |
| 1. Nbaum, G.et al.(2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.Int Immunol. 18: 1413-9.2. Steinberg, F.et al.(2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.J Cell Biol. 197 (2): 219-30.3. Zanin-Zhorov, A.et al.(2003) T cells respond to heat shock protein 60 via TLR2: activation of adhesion and inhibition of chemokine receptors.FASEB J. 17: 1567-9. |
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